Expressed sequence tags refers to

Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. It only takes a minute to sign up. Let me give it my best shot. Here's my concern: I've seen ESTs described as "one-shot" or "single-pass" sequences, which I interpret to mean a single sequence read. However, they are also typically described as being hundreds of base pairs in length Wikipedia claims about bp in length maxwhich currently limits this definition to reads derived from Sanger-style sequencing.

Does this mean that a short read derived from a high-throughput sequencing platform is not an EST? Is this distinction based on read length? Due to technical limitations, short reads are often assembled before they are used for other downstream analysis. I have sometimes heard the contigs belonging to these assemblies as ESTs, but this doesn't seem to be consistent with the previous definitions. Is our terminology becoming inconsistent due to the rapid improvements in sequencing technology, or am I missing or misinterpreting something?

The quick answer is that you are misinterpreting ESTs. They are obtained by collecting and sequencing any mature i. Well, it was living until the experiment was performed at any rate :. The term EST is not applicable to sequences derived from genomic data. It only applies to expressed sequences. Short reads are not ESTs because we have no information on whether that sequence is actually expressed in a living cell. You could apply the term to RNAseq reads depending on the experimental procedure involved but that is not what most people would understand by the term.

In summary, the operative term of EST is "expressed". You cannot call anything an EST if you do not have evidence that it is actively expressed in a living cell.

I think what you are witnessing being subject to? I put the caveat in that you are being subjected to this because I suspect the people referring to the "things" that a second-generation-sequencing protocol generates as "ESTs" are using a familiar word to describe something that might be unfamiliar to them.

I guess it's i construction; ii platform; iii throughput; and iv relative "quantitativeness". If you have a reference genome, I think assembling RNAseq reads before downstream analysis alignment? Sign up to join this community. The best answers are voted up and rise to the top. Asked 8 years, 4 months ago. Active 8 years, 4 months ago. Viewed 4k times.

Improve this question. Daniel Standage Daniel Standage 5, 3 3 gold badges 25 25 silver badges 61 61 bronze badges. Active Oldest Votes. Improve this answer. Of course, in general short reads can be genomic if you're sequencing the genome or transcriptomic if you're doing RNAseq analysis or something similar. Such transfrags do not, again in my personal opinion, qualify as ESTS. RNA-seq libraries are constructed from "living cells" as well, so what's the difference here?

Capturing these reads are evidence enough that they are expressed noise control required, obviously Also: I suspect "mature mRNAs" are captured for "EST-ifying" much the same way they are captured for constructing a highthroughput sequencing library via oligo-dTs to prime the polyA tail Can you clarify those two points a bit?

Tagpuan sa nobelang gapo

Yes RNAseq reads come from living cells. In my answer I just say that "most people" would not call the ESTs.Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly.

Related topics. Gene Library Sequence Tagged Sites. Papers overview Semantic Scholar uses AI to extract papers important to this topic. Highly Cited. MAKER: an easy-to-use annotation pipeline designed for emerging model organism genomes.

Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Expressed sequence tags: alternative or complement to whole genome sequences?

Philips straightener curler and crimper

Generation and initial analysis of more than 15, full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences…. Genome-wide detection of alternative splicing in expressed sequences of human genes. Computational and experimental analysis of microsatellites in rice Oryza sativa L.

A total of Microarrays of cells expressing defined cDNAs. The P superfamily: update on new sequences, gene mapping, accession numbers, early trivial names of enzymes, and nomenclature. We provide here a list of P genes and 12 putative pseudogenes that have been characterized as of December 14, By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License.GenBank 1 Januaryall species.

The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately to nucleotides. Alternatively, if the genome of the organism that originated the EST has been sequenced, one can align the EST sequence to that genome using a computer.

expressed sequence tags refers to

The current understanding of the human set of genes as of [update] includes the existence of thousands of genes based solely on EST evidence. In this respect, ESTs have become a tool to refine the predicted transcripts for those genes, which leads to the prediction of their protein products and ultimately of their function.

Moreover, the situation in which those ESTs are obtained tissue, organ, disease state - e. ESTs contain enough information to permit the design of precise probes for DNA microarrays that then can be used to determine gene expression profiles.

Some authors use the term "EST" to describe genes for which little or no further information exists besides the tag.

Inteams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of such in bacterial plasmids.

Inthe idea of selecting random or semi-random clones from such a cDNA library for sequencing was explored by Greg Sutcliffe and coworkers. InPutney et al. The dbEST is a division of Genbank established in Because of the way ESTs are sequenced, many distinct expressed sequence tags are often partial sequences that correspond to the same mRNA of an organism.

In an effort to reduce the number of expressed sequence tags for downstream gene discovery analyses, several groups assembled expressed sequence tags into EST contigs. Constructing EST contigs is not trivial and may yield artifacts contigs that contain two distinct gene products. When the complete genome sequence of an organism is available and transcripts are annotated, it is possible to bypass contig assembly and directly match transcripts with ESTs.

This approach is used in the TissueInfo system see below and makes it easy to link annotations in the genomic database to tissue information provided by EST data. High-throughput analyses of ESTs often encounter similar data management challenges. Similarly, disease conditions for the tissue are not annotated in a computationally friendly manner.

For instance, cancer origin of a library is often mixed with the tissue name e. The TissueInfo project was started in to help with these challenges. From Wikipedia, the free encyclopedia. National Center for Biotechnology Information. Jun Jan Nucleic Acids Res.

Emiway mp3 song download pagalworld 320kbps

Appl Bioinform. BMC Bioinformatics. Hidden categories: Webarchive template wayback links Articles containing potentially dated statements from All articles containing potentially dated statements.

Namespaces Article Talk. Views Read Edit View history. Help Learn to edit Community portal Recent changes Upload file. Download as PDF Printable version.Biology Stack Exchange is a question and answer site for biology researchers, academics, and students.

It only takes a minute to sign up. What are ESTs exactly? Why are they so instrumental in genomic research? I therefore start by dealing with the source of the confusion.

expressed sequence tags refers to

When used in this way there is no implication that even the length of this is accurately known, never mind the identity and linear arrangement of the nucleotides.

The second use of the word sequence noun is the identity and order of nucleotides in a linear nucleic acid, with the corresponding verb meaning to determine this.

Because today it is so cheap and quick to determine the sequence of a nucleic acid fragment, the student may not realize what a relatively slow and expensive undertaking it was in the last century.

dictyBase Help: Glossary Terms

The second source of confusion seems to be with the cDNA clones. This implies the poster is thinking in terms of individual cDNA clones in which the inserted cDNA fragment has been characterized — restriction mapped, perhaps sequenced and the protein product even identified. This is the approach adopted when the scientific problem is to identify the mRNA or gene encoding a particular protein — actin, beta-globin, etc.

Chauvinism meaning in urdu

This is not the case with ESTs. Here the only characterization performed on a cDNA clone is a single DNA sequence determined historically by the Sanger method from a primer position in the cloning vector.

This is because the focus is on producing a bulk library of DNA sequences. Despite its uncharacterized nature, possible redundancy, limited size perhaps nt and likelihood of errors it is useful in combination with many others from the same tissue as providing a reference to genes that are expressed in that tissue.

One presumably just isolated total DNA from the recombinants in the M13 plaques and ran a single sequence determination from the primer site. And this was because one did not know which EST would be useful, but a collection or library of ESTs would likely contain some that would be useful.

Expressed Sequence Tag

Only 3 per cent of the human genome codes for proteins… the most important part of the genome is the protein coding genes and that sequencing these will give the largest return of biological information…. After pointing out his own scepticism towards the approach he briefly mentioned one important historical issue:.

Given these disincentives, why have tens of millions of dollars been spent on ESTs? After a brief exploratory phase… a major driving force was the hope and the fear that ESTs could be used to patent any gene that had been tagged.

Attempts to patent genes via ESTs raised the spectre of an international trade war and the echoes are still reverberating around courts in the United States. It was hoped that large-scale EST projects would define the broad limits on the number of genes and the types of genes in the genome and provide a snapshot of their expression patterns. It was also expected that enough sequence information would be generated to be able to recognize new members of gene families of biological and commercial interest.

Sign up to join this community. The best answers are voted up and rise to the top. Ask Question. Asked 3 years, 10 months ago. Active 3 years, 10 months ago.

Expressed Sequence Tags (ESTs)

Viewed times. Improve this question. Active Oldest Votes. So, what are ESTs and how are they generated? Only 3 per cent of the human genome codes for proteins… the most important part of the genome is the protein coding genes and that sequencing these will give the largest return of biological information… After pointing out his own scepticism towards the approach he briefly mentioned one important historical issue: Given these disincentives, why have tens of millions of dollars been spent on ESTs?

Cask n flagon marshfield brunch

No longer, I hope. Goodfellow then turned to the science: It was hoped that large-scale EST projects would define the broad limits on the number of genes and the types of genes in the genome and provide a snapshot of their expression patterns.

Improve this answer. David David These are used in human Genome Project. Questions from NEET Choose the right one among the statements given below Reproductive Health.

Select the correct group of biocontrol agents Microbes in Human Welfare. Which of the statements given below is not true about formation of Annual Rings in trees? Anatomy of Flowering Plants. Variations caused by mutation, as proposed by Hugo de Vries, are: Evolution.

Select the hormone-releasing Intra-Uterine Devices. Reproductive Health. Which of the following glucose transporters is insulin-dependent? Column - 1 Column - II a Saprophyte i Symbiotic association of fungi with plant roots b Saprophyte ii Decomposition of dead organic materials c Lichens iii Living on living plants or animals d Mycorrhiza iv Symbiotic association of algae and fungi.

Consider the following statements : A Coenzyme or metal ion that is tightly bound to enzyme protein is called prosthetic group. B A complete catalytic active enzyme with its bound prosthetic group is called apoenzyme. Select the correct option. Questions from Molecular Basis of Inheritance. Nucleosomes were discovered by. Euchromatin has a thickness of. RNA enzyme, ribonuclease-P helps in separating from primary transcript. The distance between two ribosomes of a polyribosome is.

Inducible operon shows.

Whatsapp stickers tamil download jio phone

Control of gene expression takes place at the level of. Biology Most Viewed Questions. Match the following columns and select the correct option. In which of the following techniques, the embryos are transferred to assist those females who cannot conceive? Identify the wrong statement with reference to the gene T that controls ABO blood groups.At first you don't notice this because there is only 1 number on each page. But as you advance through the book you notice that the numbers keep advancing by 1 every time you turn the page.

SearchSort byTop ratedMost recentTop ratedFilter byAll reviewersVerified purchase onlyAll reviewersAll stars5 star only4 star only3 star only2 star only1 star onlyAll positiveAll criticalAll starsAll formatsFormat: PaperbackAll formatsText, image, videoImage and video reviews onlyText, image, videoThere was a problem filtering reviews right now.

Was this review helpful to you. Yes NoCommentInsert product linkPaste the product's web address below:Product not found. Only products offered on Amazon can be linked. SelectWhat are product links. To insert a product link, follow these steps:6.

Your message will not be posted. Please see our guidelines regarding objectionable content.

Subscribe to RSS

You must purchase at least one item from Amazon to post a commentA problem occurred while submitting your comment. Guidelines Sign in to comment Showing 0 commentsSort by: Newest Oldest There was a problem loading comments right now. If you look closely, you will note that every tenth digit or so is just a repeat of the last digit and every hundredth or so is a just the same digit repeated three times.

A sampling of this "work":Page 36 - Line 6 - 15 characters in should be 5, not 4. Page 99 - Line 18 - first three characters should be "453" not "345".

Page 145 - Line 2 - 7th and 19th characters transposed. Page 190 - Whole line of numbers omitted betwen 6th and 7th lines. Pages 210 and 211 - Two sections appear quasi-randomized, instead of randomized. Also, if you stare at it long enough, you can decode something around page 300 about Jody Foster and J.

Salinger giving me some sort of instructions. I'm going to stay up another couple nights staring at this to see if I can make out anything further. However, something kept nagging at me - something I couldn't put my finger on.

I knew that I had seen all this somewhere before, I just couldn't place it. I was thinking Dan Brown, but I think the Di Vinci Code had something to do with painting or the Vatican or something like that.

Maybe that Oprah Book Club guy who got caught lying, but that was kinda a different thing too. I let it go, but it just kept eating at me.

I knew I had seen it before, I just knew it. About a month later, I decided to bring the book along to the Rhode Island coast so that I'd have something to thumb through on the beach. As I battled livered kelp from between my toes with the murmur of low tide brushing the pebbled shore on the wind, a revelation hit me between the eyes like a Mack truck into a kindergarten. The authors (if you can call them that) had just cut-and-pasted straight out of the original work. Ringeron March 13, 2014Format: Paperback"A Million Random Digits".

They only used 10, and just kept repeating them in different combinations. I find that the first copy perfectly predicts what the numbers will be in the second copy. In my copy of the book, all of the puzzles were already filled in which I find really annoying and what is worse, most of them have been filled in wrongly. I have been through the whole book really carefully and only found seven puzzles that had been filled out correctly.

Well, making the best of a bad job, I am now going through the book trying to correct all of the faulty puzzles and I will then submit my corrections. Perhaps a second edition will be more useful. I did find last week's winning lottery numbers on page 18 though.

It was still better than Life of Pi which, aside from being irrational, included no estimations of Pi at all.PauliMSV Duisburg SV Sandhausen - Arminia BielefeldSV SandhausenArminia Bielefeld BSC Young Boys - Grasshopper-ClubBSC Young BoysGrasshopper-Club FC Zurich - FC LuzernFC ZurichFC Luzern FC Lugano - FC Lausanne-SportFC LuganoFC Lausanne-Sport Aalborg - FC CopenhagenAalborgFC Copenhagen Brondby - AGFBrondbyAGF FC Nordsjaelland - HobroFC NordsjaellandHobro Odense - SilkeborgOdenseSilkeborg Cordoba - Rayo VallecanoCordobaRayo Vallecano Tenerife - ReusTenerifeReus Oviedo - OsasunaOviedoOsasuna Lugo - Cultural LeonesaLugoCultural Leonesa Gimnastic Tarragona - Sevilla AtleticoGimnastic TarragonaSevilla Atletico Olympiakos Nicosia - A.

Kition Larnaca Nea Salamis - ArisNea SalamisAris HNK Rijeka - NK Slaven BelupoHNK RijekaNK Slaven Belupo NK Lokomotiva Zagreb - HNK Hajduk SplitNK Lokomotiva ZagrebHNK Hajduk Split Jorge Wilstermann - BloomingJorge WilstermannBlooming Nacional Potosi - BolivarNacional PotosiBolivar The Strongest - Real PotosiThe StrongestReal Potosi Guabira - Oriente PetroleroGuabiraOriente Petrolero San Jose - Sport Boys WarnesSan JoseSport Boys Warnes MFK Ruzomberok - Slovan BratislavaMFK RuzomberokSlovan Bratislava Antofagasta - U.

Catolica San Luis - Audax ItalianoSan LuisAudax Italiano Palestino - Santiago WanderersPalestinoSantiago Wanderers Curico Unido - O HigginsCurico UnidoO Higgins Maribor - AluminijMariborAluminij NK Krsko - ND GoricaNK KrskoND Gorica Golden Arrows - Mamelodi SundownsGolden ArrowsMamelodi Sundowns Bloem Celtic - Free State StarsBloem CelticFree State Stars SCR Altach - Austria ViennaSCR AltachAustria Vienna Wolfsberger AC - Red Bull SalzburgWolfsberger ACRed Bull Salzburg Lech Poznan - Cracovia KrakowLech PoznanCracovia Krakow Wisla Krakow - Wisla PlockWisla KrakowWisla Plock Pogon Szczecin - Zaglebie LubinPogon SzczecinZaglebie Lubin Mohun Bagan - Churchill BrothersMohun BaganChurchill Brothers CD Olimpia - Lobos UPNFMCD OlimpiaLobos UPNFM Bari - PalermoPalermo FAR Rabat - Renaissance BerkaneFAR RabatRenaissance Berkane Hassania Agadir - FUS RabatHassania AgadirFUS Rabat Racing De Casablanca - Chabab Rif HoceimaRacing De CasablancaChabab Rif Hoceima Zira - Neftchi BakuZiraNeftchi Baku Keshla FC - KapazKeshla FCKapaz FK Borac Banja Luka - Siroki BrijegFK Borac Banja LukaSiroki Brijeg Zeljeznicar Sarajevo - Radnik BijeljinaZeljeznicar SarajevoRadnik Bijeljina NK Vitez - FK SarajevoNK VitezFK Sarajevo 1:0EAntigua GFC - Coban Imperial1Antigua GFC0ECoban Imperial Arouca - LeixoesAroucaLeixoes Nacional - Gil VicenteNacionalGil Vicente Sp.

Covilha - Uniao MadeiraSp. CovilhaUniao Madeira Oliveirense - PenafielOliveirensePenafiel Academico Viseu - SC Braga BAcademico ViseuSC Braga B FC Porto B - Guimaraes BFC Porto BGuimaraes B Varzim - Cova De PiedadeVarzimCova De Piedade Benfica B - Sporting CP BBenfica BSporting CP B Denizlispor - SamsunsporDenizlisporSamsunspor Giresunspor - RizesporGiresunsporRizespor Manisaspor - AltinorduManisasporAltinordu Saba Qom - Fajr SepasiSaba QomFajr Sepasi Mes Kerman - Iranjavan BushehrMes KermanIranjavan Bushehr Nassaji Mazandaran - Rah AhanNassaji MazandaranRah Ahan Bargh Shiraz - Khoneh Be KhonehBargh ShirazKhoneh Be Khoneh Oxin Alborz - Aluminium ArakOxin AlborzAluminium Arak Shahrdari Mahshahr - Gol GoharShahrdari MahshahrGol Gohar 3:2El Tanque Sisley - Sud America3El Tanque Sisley2HT 1:0Sud America Wanderers SC - Waitakere UnitedWanderers SCWaitakere United Hawkes Bay United - Tasman UnitedHawkes Bay UnitedTasman United Southern United - Canterbury UnitedSouthern UnitedCanterbury United Melbourne City - C.

Coast Mariners FCMelbourne CityC. Coast Mariners FC Panserraikos - VeriaPanserraikosVeria Doxa Dramas - Aris ThessalonikisDoxa DramasAris Thessalonikis Panegialios - ErgotelisPanegialiosErgotelis Kissamikos - SpartiKissamikosSparti Trikala - Apollon Larissa FCTrikalaApollon Larissa FC Zalaegerszegi TE - Gyirmot SEZalaegerszegi TEGyirmot SE Kisvarda - Gyori Eto FCKisvardaGyori Eto FC Cegledi VSE - MTK BudapestCegledi VSEMTK Budapest Bekescsaba 1912 - Szeged 2011Bekescsaba 1912Szeged 2011 Budaorsi SC - NyiregyhazaBudaorsi SCNyiregyhaza Siofok - MTE 1904SiofokMTE 1904 Szolnoki MAV FC - SoroksarSzolnoki MAV FCSoroksar VAC - KazincbarcikaiVACKazincbarcikai Csakvar - Soproni VSECsakvarSoproni VSE Mumbai City - Chennaiyin FCMumbai CityChennaiyin FC Jamshedpur FC - Pune CityJamshedpur FCPune City Catania - MateraCataniaMatera Paganese - LeccePaganeseLecce Fondi - AkragasFondiAkragas Monopoli - TrapaniMonopoliTrapani Siracusa - Juve StabiaSiracusaJuve Stabia Fidelis Andria - RendeFidelis AndriaRende Casertana - BisceglieCasertanaBisceglie Cosenza - Virtus FrancavillaCosenzaVirtus Francavilla Reggina - Sicula LeonzioRegginaSicula Leonzio Los Andes - Nueva ChicagoLos AndesNueva Chicago Boca Unidos - AgropecuarioBoca UnidosAgropecuario Deportivo Moron - Guillermo BrownDeportivo MoronGuillermo Brown Gimnasia Jujuy - San Martin TucumanGimnasia JujuySan Martin Tucuman Toronto FC - Seattle SoundersToronto FCSeattle Sounders Racing Santander - Real Sociedad BRacing SantanderReal Sociedad B CD Vitoria - CD LealtadCD VitoriaCD Lealtad Sporting Gijon B - Real UnionSporting Gijon BReal Union Barakaldo - Caudal DeportivoBarakaldoCaudal Deportivo Mirandes - Arenas ClubMirandesArenas Club Carrarese - LivornoCarrareseLivorno Alessandria - PistoieseAlessandriaPistoiese Arzachena - Pro PiacenzaArzachenaPro Piacenza Gavorrano - GIANA ErminioGavorranoGIANA Erminio Monza - Cuneo Viterbese Castrense - OlbiaViterbese CastrenseOlbia Pisa - ArezzoArezzo Prato - PontederaPratoPontedera FC Wohlen - Servette FCFC WohlenServette FC Rapperswil - FC AarauRapperswilFC Aarau Bucaspor Izmir - KastamonusporBucaspor IzmirKastamonuspor Mersin Idman Yurdu - InegolsporMersin Idman YurduInegolspor BB Bodrumspor - EyupsporBB BodrumsporEyupspor Hatayspor - AmedsporHataysporAmedspor Menemen Belediye - Etimesgut Bld.

Spor Tokatspor - Sancaktepe BelediyeTokatsporSancaktepe Belediye Tuzlaspor - Kocaeli BirliksporTuzlasporKocaeli Birlikspor F91 Dudelange - Union Titus PetangeF91 DudelangeUnion Titus Petange FC Differdange 03 - US EschFC Differdange 03US Esch AS Jeunesse Esch - Fola EschAS Jeunesse EschFola Esch Epinal - Blanc MesnilEpinalBlanc Mesnil Double Flower - Kwun TongDouble FlowerKwun Tong Dreams Metro Gallery - Hoi KingDreams Metro GalleryHoi King Shatin - Eastern DistrictShatinEastern District Sun Hei SC - Mutual FCSun Hei SCMutual FC Tung Sing FC - Wing YeeTung Sing FCWing Yee Wong Tai Sin - Tai ChungWong Tai SinTai Chung HKFC - WanchaiHKFCWanchai Melbourne City (w) - Canberra United (w)Melbourne City (w)Canberra United (w) Brisbane Roar FC (w) - Newcastle Jets FC (w)Brisbane Roar FC (w)Newcastle Jets FC (w) BC Rangers - Kitchee SCBC RangersKitchee SC Lee Man FC - Southern District RSALee Man FCSouthern District RSA SC Freiburg (w) - FF USV Jena (w)SC Freiburg (w)FF USV Jena (w) Frankfurt (w) - SGS Essen (w)Frankfurt (w)SGS Essen (w) Turbine Potsdam (w) - 1899 Hoffenheim (w)Turbine Potsdam (w)1899 Hoffenheim (w) Werder Bremen (w) - FC Koln (w)Werder Bremen (w)FC Koln (w) Jumilla - Ecija BalompieJumillaEcija Balompie San Fernando - MelillaSan FernandoMelilla UD Badajoz - Betis DeportivoUD BadajozBetis Deportivo Cartagena - Real LinenseCartagenaReal Linense Marbella FC - UCAM MurciaMarbella FCUCAM Murcia Extremadura - Merida UDExtremaduraMerida UD Murcia - CF Lorca DeportivaMurciaCF Lorca Deportiva Fuenlabrada - CoruxoFuenlabradaCoruxo San Sebastian De Los Reyes - Rayo MajadahondaSan Sebastian De Los ReyesRayo Majadahonda Union Adarve - PontevedraUnion AdarvePontevedra Valladolid B - Gimnastica SegovianaValladolid BGimnastica Segoviana Celta Vigo B - NavalcarneroCelta Vigo BNavalcarnero Cerceda - Deportivo FabrilCercedaDeportivo Fabril Rapido De Bouzas - TalaveraRapido De BouzasTalavera C.

PredictZ provides free football tips and predictions, free analysis, football form and statistics, the latest results and league tables and much more. Click here to set your odds preferenceWatch these live streams online, starting soon.

View bet365 live streaming disclaimerAll of these games below are available to watch for customers from Russian FederationOur betting offer alerts are updated daily to bring you all the latest deals, free bets, money back specials, and enhanced prices as they are publishedCheck out these latest upcoming predictions.

Click each game to view detailed analysis and statistics and click each league to see our full set of predictions for each league.

Would you prefer to view these predictions using fractional odds. Click here to set your odds preference HomePredictionsResultsLeague TablesStatisticsFormBet Offers Champions LeagueEuropa LeagueConfederations CupCopa AmericaInternationalInternational U21World Cup 2018Africa Cup Of NationsEnglandEng. Choose soccer league and you will find statistics, picks, tables and information for all your betting needs.

All football information on this site is free. You can choose a football game by date or select league from the country list. For detailed information about match click into score link. Don't let gambling become a problem in your life. Check our responsible gambling page for more info. Bundesliga18:00 Augsburg-:-Hertha Berlin 1. They are the ratio of the full payout to the stake, in a decimal format. Decimal odds of 2.

expressed sequence tags refers to

Types of bets - 1, X, 2 are your primary and most common bets. Our goal is to be informative, objective and reliable. But there is no guarantee that, even with the best advice available, you will become a successful punter because not everyone has what it takes to be a successful punter.

thoughts on “Expressed sequence tags refers to

Leave a Reply

Your email address will not be published. Required fields are marked *